Antibacterial Activity of Ecoenzymes with Various Dilutions Against Escherichia Coli

Authors

  • Najla Lubis Universitas Pembangunan Panca Budi
  • Rizki Damayanti Universitas Pembangunan Panca Budi
  • Sheila Wardani Universitas Pembangunan Panca Budi

Keywords:

Antibacterial, Ecoenzymes, Escherichia Coli, Dilution

Abstract

Garbage enzyme (GE), also known as ecoenzyme (EE), is a biotechnology product made by fermenting water, organic components from waste and agricultural products, and carbohydrates. Finding the ideal concentration of ecoenzyme as an antibacterial agent is the goal of this study. Organic components from waste pineapple, orange, papaya, star fruit, kuni, and mango peels (Bioz1) as well as waste pineapple, orange, banana tubers, noni, and kuni (Bioz2) are used to make ecoenzymes. Escherichia coli bacteria that are gram-negative are cultured using the disc method. Four different dilution ratios are used: 0; 1:10; 1:50; and 1:100. The best dilution variation to inhibit Escherichia coli bacteria, according to the data, was a 1:10 ratio.

References

K. Warsito, L. Yamurni, Ri. Pradinata, L. E. B. Tamba, and W. S. Siregar, Buddaya Tanaman Tahunan Dengan Ekoenzim, 1st ed. Medan: BUDIDAYA TANAMAN TAHUNAN DENGAN EKOENZIM Kabul Warsito, S.Si., M.Si Lily Yamurni Rio Pradinata Listina Elisabet Br. Tamba Winda Sari Siregar TAHTA MEDIA GROUP, 2023.

N. Lubis, M. Wasito, L. Marlina, R. Girsang, and H. Wahyudi, “Respon Pemberian Ekoenzim dan Pupuk Organik Cair Terhadap Pertumbuhan dan Produksi Bawang Merah (Allium ascalonicum L.),” Agrium, vol. 25, no. 2, pp. 107–115, 2022, doi: 10.30596/agrium. v25i2.10354 107.

N. Lubis, M. Wasito, L. Marlina, S. T. Ananda, and H. Wahyudi, “Potensi ekoenzim dari limbah organik untuk meningkatkan produktivitas tanaman,” no. Hasanah 2021, pp. 978–979, 2022.

A. D. Luta, M. Siregar, F. H. Syam, Y. Feruzi, and J. Syafridawani, “Efektivitas Pemberian Media Tanam dan Ekoenzim Pada Pertumbuhan Bawang Merah (Allium ascalonicum L .),” in Seminar Nasional UNIBA Surakarta, Surakarta, 2022, pp. 275– 277.

M. Wasito, “Training on Making Eco Enzymes from Fruit Waste in Suka Damai Village, Kuala Sub-District, Langkat District,” vol. 2, no. 2, pp. 232–241, 2023.

T. Hakim, R. R. A. Tarigan, and Sulardi, “PENGGUNAAN PUPUK CAIR ORGANIK DALAM MENINGKATKAN PERTUMBUHAN DAN PRODUKSI BAWANG

MERAH (Alium ascalonicum) Var. Sanren F1,” vol. 25, no. 4, pp. 3388–3396, 2023.

R. B. Ginting, D. J. S. Siregar, Warisman, and R. R. Putra, “CRUDE PROTEIN CONTENT, CRUDE FAT AND CRUDE FIBER FERMENTED CASSAVA TUBER PEEL (KUUK) WITH ECO ENZYMES,” J. Innov. Res. Knowl., vol. 3, no. 5, pp. 1109–1114, 2023.

A. H. Ramadani, R. Karima, and R. S. Ningrum, “Antibacterial Activity of Pineapple Peel (Ananas comosus) Eco-enzyme Against Acne Bacterias (Staphylococcus aureus and Prapionibacterium acnes),” Indo. J. Chem. Res., vol. 9, no. 3, pp. 201–207, 2022, doi: 10.30598//ijcr.

N. Ginting and L. Prayitno, “Dilution of Eco Enzyme and Antimicrobial Activity Against Staphylococcus aureus Animal Production Study Program, Faculty of Agriculture, Universitas Sumatera Utara, Padang,” pp. 123–128, 2022, doi: 10.33772/jitro.v9i1.19705.

J. A. Donaghy et al., “Relationship of sanitizers, disinfectants, and cleaning agents with antimicrobial resistance,” J. Food Prot., vol. 82, no. 5, pp. 889–902, 2019, doi: 10.4315/0362-028X.JFP-18-373.

H. Mulyadi, Z. A. Kamila, E. Susanti, and N. Y. Haryono, “Optimasi Waktu Fermentasi Ekoenzim dari Limbah Kulit Kopi dengan Sumber Karbon Molase,” Live Appl. Sci., vol. 1, pp. 41–48, 2022.

Z. A. Kamila, H. Mulyadi, Suharti, and N. Y. Haryono, “Optimasi Pembuatan Ekoenzim dari Limbah Kulit Kopi dan Pepaya,” Pros. Semin. Bioteknol. Nas., vol. 1,

pp. 129–137, 2022.

N. Ginting, H. Hasnudi, and Y. Yunilas, “Eco-enzyme Disinfection in Pig Housing as an Effort to Suppress Esherechia coli Population,” J. Sain Peternak. Indones., vol. 16, no. 3, pp. 283–287, 2021, doi: 10.31186/jspi.id.16.3.283-287.

W. W. Davis and T. R. Stout, “Disc plate method of microbiological antibiotic assay. I. Factors influencing variability and error.,” Appl. Microbiol., vol. 22, no. 4, pp. 659– 665, 1971, doi: 10.1128/aem.22.4.659-665.1971.

Rusdianasari, A. Syakdani, M. Zaman, F. F. Sari, N. P. Nasyta, and R. Amalia, “Production of Disinfectant by Utilizing Eco-enzyme from Fruit Peels Waste,” Int. J. Res. Vocat. Stud., vol. 1, no. 3, pp. 01–07, 2021, doi: 10.53893/ijrvocas. v1i3.53.

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Published

2024-10-21

How to Cite

Lubis, N., Damayanti, R., & Wardani, S. (2024). Antibacterial Activity of Ecoenzymes with Various Dilutions Against Escherichia Coli. International Conferance Of Digital Sciences And Engineering Technology , 1(1), 122–127. Retrieved from https://proceeding.pancabudi.ac.id/index.php/ICDSET/article/view/169